Fig 1: Nanog deletion triggers gastric differentiation in KL model A, BHuman (A) and KLN murine (B) lung sections were stained with H&E or with Periodic acid–Schiff (PAS) and Alcian blue (AB). Scale bar, 50 µm.CStatistical analysis of mucinous tumor numbers (no.) in KL (n = 7) or KLN (n = 7) mice. Significance was calculated by two-tailed unpaired Student’s t-test with Welch’s correction. Results were shown as mean. **P = 0.0066.DRepresentative pictures of lung tumors at 4 weeks and 8 weeks post-Ad-Cre infection in KLN mice. Scale bar, 50 µm.EStatistical analysis of IMA ratio at 4 and 8 weeks post-Ad-Cre treatment (n = 5 per group). Results were shown as mean ± SEM.FStatistical analysis of ratio of mucinous phenotype in AAH, AD, and ADC (n = 5 per group). Results were shown as mean ± SEM. Significance was calculated by one-way ANOVA with Dunnett’s multiple comparisons test. *P = 0.0215.GqPCR analysis of stomach-restricted gene set between KL ADC and KLN mucinous tumor sections (n = 3 or 4 per group). Results were shown in a box and whisker plot with the minimum value, first quartile, median, third quartile, and maximum value. The box represented the 50% of the central data, with a line inside represented the median. The significance was calculated by two-tailed unpaired Student’s t-test with Welch’s correction. *P = 0.0260 (Hnf4a), *P = 0.0275 (Vsig1), *P = 0.0122 (Gkn3), *P = 0.0324 (Ctse), **P = 0.0084 (Onecut2).HRepresentative histological and IHC staining for HNF4a, GKN1, NKX2-1, MUC5AC, CK20 in lung tumors derived from ADC and IMA. Scale bar, 50 µm.